Composite

Part:BBa_K2651000:Design

Designed by: Catherosette Meas   Group: iGEM18_ULaVerne_Collab   (2018-10-12)


Strong Promoter(J23110), RBS (B0034), and PETase CDS with Amil-CP reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design this sequence to be quickly characterizable (If the vector is working, the protein would be seen as blue). We also made an exact replicate of mutant Petase to be compared side by side to Petase.


Source

Came from a finding by Yoshida et al 2016 from Ideonella sakiensis. We ordered the gene sequence from IDT and connected to two Biobrick parts: J23110 and K1357009 that we receive from the registry.

References